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KMID : 0811720040080000191
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Korean Journal of Physiology & Pharmacology
2004 Volume.8 No. 0 p.191 ~ p.0
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An Inhibitory Mechanism of Serotonin in Exocrine Secretion of the Isolated Perfused Rat Pancreas
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Lee Kyoung-Hee
Koh Hyo-Jin
Kang Jung-I
Kwon Hyeok-Yil
Lee Yun-Lyul
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Abstract
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We previously reported that electrical field stimulation (EFS)-evoked pancreatic volume flow and amylase output further elevated by spiperone hydrochloride, serotonin (5-HT) antagonist, in the isolated rat pancreas. These findings represent that endogenous serotonin released by EFS in the intrapancreatic serotonergic nerves inhibited pancreatic exocrine secretion. Thus, this study was aimed to investigate that action of exogenous serotonin and an action mechanism on the pancreatic exocrine secretion. The pancreas isolated and continually perfused with modified Krebs-Henseleite solution containing 5.6 or 18 mM glucose, 0.1% BSA and 3% dextran via the celiac and superior mesenteric arteries at a flow rate of 1.2 ml/min. To find actions of exogenous serotonin, serotonin (2μM)was administered to cholecystokinin (CCK)-stimulated pancreatic exocrine secretion with or without endogenous insulin. All rats were fasted for 24h before the preparations of isolated pancreas. Pancreatic volume flow and amylase output were determined from pancreatic juice collected in 15 min samples. CCK elevated pancreatic volume flow and amylase output in the isolated perfused rat pancreas. Endogenous insulin released by 18 mM glucose potentiated the action of CCK. CCK-stimulated pancreatic volume flow and amylase output were not change by exogenous serotonin under 5.6 mM glucose background. However, exogenous serotonin significantly inhibited CCK-stimulated pancreatic volume flow and amylase output under 18 mM glucose background (p＜0.05). Exogenous serotonin significantly (p＜0.05) inhibited insulin concentrations in the portal effluents. These observations indicate that serotonin play a inhibitory role in pancreatic exocrine secretion and insulin may be involved the inhibitory actions. This work was supported by grant (R05-2004-000-11637-0) from Ministry of Science & Technology.
Source: Korean Journal of Physiology & Pharmacology.2004 Oct;8(Suppl I):S140-S140
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KEYWORD
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Serotonin, Electrical field stimulation, Exocrine secretion, Pancreas, Rat
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